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骨骼肌的线粒体分离
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JoVE Journal Biology
Mitochondrial Isolation from Skeletal Muscle
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骨骼肌的线粒体分离

DOI:
10.3791/2452-v

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09:45 min

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March 30, 2011

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, ,
1Department of Physiology,University of Kentucky

Chapters

  • 00:05Title
  • 01:15Muscle Isolation/Homogenization and Mitochondrial Isolation
  • 04:13Electrode Calibration
  • 06:17Mitochondrial Respiration
  • 08:15Representative Mitochondrial Respiration Results
  • 09:15Conlcusion

Summary

Automatic Translation

Automatic Translation

这个协议描述一个过程来研究从骨骼肌中分离出的线粒体呼吸。这种方法改编自Scorrano<em>等。</em>（2007年）。线粒体的分离过程中需要约2小时。线粒体呼吸在大约1个小时就可以完成。

Tags

Mitochondrial IsolationSkeletal MuscleOrganellesMetabolic ReactionsATP SynthesisSignaling CascadesLiverBrainHeartSkeletal Muscle FunctionIsolation MethodBuffersReagentsMitochondrial Protein ConcentrationFunctional StudiesMitochondrial RespirationPolarographyClark-type ElectrodeOxygen Electrode CalibrationExperimental BufferGlutamateMalateAdenosine Diphosphate (ADP)Oligomycin

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