Journal
/
Biology
/
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

DOI:
10.3791/63336-v

•

08:11 min

•

February 10, 2022

•

, , , , ,
1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA,Universidad Pablo de Olavide, 2CIBERER,Instituto de Salud Carlos III

Chapters

  • 00:05Introduction
  • 00:53Muscle Dissection, Homogenization, and Mitochondrial Isolation
  • 04:35Preparation of the Microplate-Based Respirometric Assays
  • 06:17Results: Mitochondria Isolation from Mouse Skeletal Muscle
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.

Tags

Mitochondria IsolationRespirometric AssaysSkeletal MuscleMousePurificationRespiration AnalysisExperimental ConditionsOrganismAgingDrug TestingCancerDevelopmentPBSEDTAIB1DissectionSkeletal MusclesTibialis AnteriorExtensor Digitorum LongusGastrocnemiusSoleus

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Mitochondrial Isolation from Skeletal Muscle

Purification of Progenitors from Skeletal Muscle

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

Read Article