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从小鼠骨骼肌中分离线粒体用于呼吸测定
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
DOI:

08:11 min

February 10, 2022

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Chapters

  • 00:05Introduction
  • 00:53Muscle Dissection, Homogenization, and Mitochondrial Isolation
  • 04:35Preparation of the Microplate-Based Respirometric Assays
  • 06:17Results: Mitochondria Isolation from Mouse Skeletal Muscle
  • 07:32Conclusion

Summary

Automatic Translation

在这里,我们描述了从小鼠骨骼肌中分离线粒体的详细方法,以及随后使用基于微孔板的呼吸测定通过耗氧率(OCR)分析呼吸。该管道可用于研究多种环境或遗传干预措施对线粒体代谢的影响。

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