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1Biomedical Engineering Department,Cornell University, 2Neurosurgical Laboratory for Translational Stem Cell Research, Weill Cornell Brain Tumor Center,Weill Cornell Medical College of Cornell University, 3Cell Morphology Department,Instituto de Investigacion Principe Felipe, 4Department of Chemical and Biomolecular Engineering,Cornell University
Chapters
- 00:30Introduction
- 02:10Assembling the device
- 07:54Cell seeding and imaging
- 11:12Conclusion
Nós demonstramos que a expressão de receptores de mais de fator de crescimento epidérmico (EGFR) aumenta a motilidade das células-tronco neurais (NSCs) usando um romance agarose gel com base em dispositivo microfluídicos. Esta tecnologia pode ser facilmente adaptável a outros sistemas de células de mamíferos, onde fontes de células são escassos, como células-tronco neurais, ea volta em torno do tempo é crítica.
Tags
Neural Stem CellsMotilityAgarose Gel-based Microfluidic DeviceMicrofluidic TechnologyCell-based AssaysMicroenvironmentConventional Microfabrication TechniquesMaterialsAgarose GelCell MigrationParallel Microfluidic ChannelsPeristaltic PumpObservationChemical EnvironmentOptical MonitoringExperimental Results
