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Journal
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Cancer Research
/
医薬品評価研究のための3D腫瘍スフェロイドの作製
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JoVE Journal
Cancer Research
Generation of 3D Tumor Spheroids for Drug Evaluation Studies
Please note that all translations are automatically generated.
Click here for the English version.
医薬品評価研究のための3D腫瘍スフェロイドの作製
DOI:
10.3791/65125-v
•
10:33 min
•
February 24, 2023
•
Wei Hou*
1,2
,
Zilin Zhang*
3,4
,
Jing Zhang
4
,
Shihui Xu
4
,
Menglan Li
,
Xiaoran Li
4
,
Zaozao Chen
3
,
Cailian Wang
2
1
Department of Oncology, School of Medicine
,
Southeast University
,
2
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering
,
Southeast University
,
3
Institute of Medical Devices (Suzhou)
,
Southeast University
,
4
Jiangsu Avatarget Biotechnology Co., Ltd.
Chapters
00:04
Introduction
00:58
Spheroid Construction
05:38
Drug Treatment and Spheroid Viability
07:09
Spheroid Observation and Deep Learning Analysis Through Images in the Drug Test
08:23
Results: Brightfield Images of NCI‐H23 Cell Spheroids Treated with AMG510, Tumor Spheroid Viability, Tumor Size and Invasiveness
10:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この記事では、3次元腫瘍スフェロイドを構築するための標準化された方法を示します。また、自動イメージングシステムを用いたスフェロイド観察と画像ベースの深層学習解析の戦略についても説明します。
Tags
3D Tumor Spheroids
Drug Evaluation
High-throughput Imaging
High-content Analysis
AMG510
NCI-H23 Cells
Anti-adhesion Reagent
Trypsin-EDTA
Cell Suspension
Cell Seeding Density
Centrifugation
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