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生成用于药物评估研究的 3D 肿瘤微球
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Cancer Research
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JoVE Journal Cancer Research
Generation of 3D Tumor Spheroids for Drug Evaluation Studies
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生成用于药物评估研究的 3D 肿瘤微球

DOI:
10.3791/65125-v

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10:33 min

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February 24, 2023

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, , , , , , ,
1Department of Oncology, School of Medicine,Southeast University, 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering,Southeast University, 3Institute of Medical Devices (Suzhou),Southeast University, 4Jiangsu Avatarget Biotechnology Co., Ltd.

Chapters

  • 00:04Introduction
  • 00:58Spheroid Construction
  • 05:38Drug Treatment and Spheroid Viability
  • 07:09Spheroid Observation and Deep Learning Analysis Through Images in the Drug Test
  • 08:23Results: Brightfield Images of NCI‐H23 Cell Spheroids Treated with AMG510, Tumor Spheroid Viability, Tumor Size and Invasiveness
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

本文演示了一种构建三维肿瘤球体的标准化方法。还描述了使用自动成像系统进行球体观察和基于图像的深度学习分析的策略。

Tags

3D Tumor SpheroidsDrug EvaluationHigh-throughput ImagingHigh-content AnalysisAMG510NCI-H23 CellsAnti-adhesion ReagentTrypsin-EDTACell SuspensionCell Seeding DensityCentrifugation

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