/

/

使用创新的无异种方法进行人类治疗，分离和培养人脂肪来源的干细胞

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Please note that all translations are automatically generated. Click here for the English version.

使用创新的无异种方法进行人类治疗，分离和培养人脂肪来源的干细胞

DOI:

10.3791/65104-v

•

06:00 min

•

February 03, 2023

•

Martino Guiotto*^1,4, Silvia Palombella*², Stefania Brambilla, Lee Ann Applegate, Mathis Riehle, Andrew Hart, Wassim Raffoul, Pietro Giovanni di Summa

¹Department of Plastic, Reconstructive and Hand Surgery,Centre Hospitalier Universitaire Vaudois (CHUV, ²Cell and Tissue Engineering Lab,IRCCS Ospedale Galeazzi Sant’Ambrogio, ³Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine,Lausanne University Hospital, ⁴Centre for the Cellular Microenvironment,University of Glasgow

Chapters

00:04Introduction
01:04Human Adipose-Derived Stem Cells (hASCs) Isolation from Adipose Tissue
02:21hASC Culture, Cryopreservation, and Storage
04:25Results: Assessment of Human Platelet Lysate (hPL)-Induced hASCs
05:22Conclusion

Summary

Automatic Translation

在细胞疗法制备/操作步骤中引入的异源性（化学或动物源性）产品与宿主患者免疫反应性和致病性传播的风险增加有关。这里描述了一种完整的无异种方法，用于分离和体外扩增人脂肪来源的干细胞。

Tags

Adipose-derived Stem Cells Human Therapy Xenogeneic-free Method Cell Isolation Cell Expansion Peripheral Nerve Repair Cell Culture Sterility Micro Dissection Cell Attachment Cell Confluency Cell Passage Trypsinization Cell Collection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在干细胞移植在体外模拟缺血/再灌注模型

验尸报告组织心肌细胞核分离

基因治疗的载体和干细胞视网膜下注射到老鼠的眼睛

人牙髓干细胞的分离，鉴定和比较性恒牙使用两种不同的方法，得出

来自患者的细胞培养和分离的CD133<sup> +</sup>假定的肿瘤干细胞的恶性黑色素瘤

分离，培养和成像胎儿胰腺细胞集群

癌症干细胞从人类前列腺癌样本隔离

丰富的人力细胞系化疗耐药性卵巢癌干细胞

分离，冻存和文化的人羊膜上皮细胞的临床应用

人垂体腺瘤细胞的三维海藻酸钠珠文化

Read Article