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マウスおよびヒト涙腺オルガノイドの樹立、維持、分化、遺伝子操作、移植
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
Please note that all translations are automatically generated.
Click here for the English version.
マウスおよびヒト涙腺オルガノイドの樹立、維持、分化、遺伝子操作、移植
DOI:
10.3791/65040-v
•
10:49 min
•
February 03, 2023
•
Marie Bannier-Hélaouët
2
,
Maarten H. Geurts
2
,
Jeroen Korving
,
Harry Begthel
,
Hans Clevers
2
1
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW)
,
University Medical Center Utrecht
,
2
Oncode Institute
,
Hubrecht Institute
Chapters
00:04
Introduction
00:39
Organoid Derivation
03:41
Functional Swelling Assay
04:45
Organoid Electroporation
06:36
Orthotopic Transplantation of Human Lacrimal Gland Organoids
08:17
Results: Establishment, Genetic Modification, Differentiation, Functional Characterization, and Transplantation of Lacrimal Gland Organoids
10:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、初代マウスおよびヒト組織に由来する涙腺オルガノイドを確立、維持、遺伝子改変、分化、機能的に特徴付け、および移植する方法を説明しています。
Tags
Lacrimal Gland Organoids
Adult Stem Cell
Regenerative Medicine
Dry Eye Disease
Extracellular Matrix
Cell Culture
Mouse
Human
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