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Journal
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Biology
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小鼠和人泪腺类器官的建立、维持、分化、遗传操作和移植
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
Please note that all translations are automatically generated.
Click here for the English version.
小鼠和人泪腺类器官的建立、维持、分化、遗传操作和移植
DOI:
10.3791/65040-v
•
10:49 min
•
February 03, 2023
•
Marie Bannier-Hélaouët
2
,
Maarten H. Geurts
2
,
Jeroen Korving
,
Harry Begthel
,
Hans Clevers
2
1
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW)
,
University Medical Center Utrecht
,
2
Oncode Institute
,
Hubrecht Institute
Chapters
00:04
Introduction
00:39
Organoid Derivation
03:41
Functional Swelling Assay
04:45
Organoid Electroporation
06:36
Orthotopic Transplantation of Human Lacrimal Gland Organoids
08:17
Results: Establishment, Genetic Modification, Differentiation, Functional Characterization, and Transplantation of Lacrimal Gland Organoids
10:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何建立,维持,基因修饰,分化,功能表征和移植源自原代小鼠和人体组织的泪腺类器官。
Tags
Lacrimal Gland Organoids
Adult Stem Cell
Regenerative Medicine
Dry Eye Disease
Extracellular Matrix
Cell Culture
Mouse
Human
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