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Chapters
- 00:05Introduction
- 01:05Bone Marrow Extraction and c-Kit+ Enrichment of Hematopoietic Stem Cell (HSC)
- 03:03Initiating Cell Cultures with c-Kit-Enriched Hematopoietic Stem and Progenitor Cells (HSPCs)
- 03:45Performing Media Changes
- 04:19Electroporating Cultured HSPCs
- 05:24Transducing Cultured HSPCs with Lentiviral Vector
- 06:17Results: Fluorescence-Activated Cell Sorting (FACS) of the c-Kit-Enriched HSCs
- 07:22Conclusion
Aquí se presenta un protocolo para iniciar, mantener y analizar cultivos de células madre hematopoyéticas de ratón utilizando expansión ex vivo a base de alcohol polivinílico, así como métodos para manipularlos genéticamente mediante transducción lentiviral y electroporación.
