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离体
聚乙烯醇基培养物中小鼠造血干细胞的扩增和遗传操作
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Biology
Ex Vivo
Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures
Please note that all translations are automatically generated.
Click here for the English version.
离体
聚乙烯醇基培养物中小鼠造血干细胞的扩增和遗传操作
DOI:
10.3791/64791-v
•
07:55 min
•
February 10, 2023
•
Hwei Minn Khoo*
1
,
Grace A. Meaker*
1
,
Adam C. Wilkinson
1
MRC Weatherall Institute of Molecular Medicine
,
University of Oxford
Chapters
00:05
Introduction
01:05
Bone Marrow Extraction and c-Kit
+
Enrichment of Hematopoietic Stem Cell (HSC)
03:03
Initiating Cell Cultures with c-Kit-Enriched Hematopoietic Stem and Progenitor Cells (HSPCs)
03:45
Performing Media Changes
04:19
Electroporating Cultured HSPCs
05:24
Transducing Cultured HSPCs with Lentiviral Vector
06:17
Results: Fluorescence-Activated Cell Sorting (FACS) of the c-Kit-Enriched HSCs
07:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是使用
离体
聚乙烯醇基扩增启动、维持和分析小鼠造血干细胞培养物的方案,以及通过慢病毒转导和电穿孔进行遗传操作的方法。
Tags
Hematopoietic Stem Cells
Ex Vivo Expansion
Genetic Manipulation
Polyvinyl Alcohol
Mouse
Bone Marrow
C-Kit
Magnetic Column Separation
Cell Culture
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