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从人眼高效、一致地生成视网膜色素上皮/脉络膜平面安装,用于组织学分析
JoVE Journal
Biology
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JoVE Journal
Biology
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Please note that all translations are automatically generated.
Click here for the English version.
从人眼高效、一致地生成视网膜色素上皮/脉络膜平面安装,用于组织学分析
DOI:
10.3791/64761-v
•
07:59 min
•
October 28, 2022
•
Davide Ortolan
,
Andrei Volkov
,
Arvydas Maminishkis
,
Ruchi Sharma
,
Kapil Bharti
1
Ocular and Stem Cell Translational Research Section, National Eye Institute
,
National Institutes of Health (NIH)
Chapters
00:04
Introduction
00:46
Silicone Mold Preparation
01:21
RPE Dissection
03:42
Staining
04:45
ReShAPE Analysis
06:17
Results: Complete Morphometric Analysis of Entire Human RPE Monolayer
07:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一种有效地将视网膜色素上皮(RPE)与人眼视网膜分离并生成整个RPE /脉络膜平板安装以用于RPE的组织学和形态学分析的方法。
Tags
Retinal Pigment Epithelium
RPE
Choroid
Flatmount
Histological Analysis
Retinal Degeneration
Dissection Technique
REShAPE Software
D-Mannitol
DPBS
PFA
Macular Region
Ciliary Margin
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