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Generation of Human Kidney Tubuloids from Tissue and Urine
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Human Kidney Tubuloids from Tissue and Urine

Generation of Human Kidney Tubuloids from Tissue and Urine

DOI:
10.3791/62404-v

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08:34 min

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April 16, 2021

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,
1Princess Máxima Center for Pediatric Oncology, 2Oncode Institute

Chapters

  • 00:04Introduction
  • 00:50Generation of Human Kidney Tubuloids From Tissue
  • 03:45Generation of Human Kidney Tubuloids From Urine
  • 05:02Expansion of Tubuloid Cultures
  • 07:01Results: Successful Generation of Human Kidney Tubuloids From Tissue and Urine
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

Human kidney tubuloid cultures represent a valuable in vitro model to study kidney physiology and disease. Tubuloids can be established from kidney tissue (healthy and diseased) as well as urine, the latter representing an easily obtainable and less invasive source of research material.

Tags

Kidney TubuloidsUrineKidney Disease ModelingKidney PhysiologyBasement Membrane ExtractCollagenaseCell Culture

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