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Journal
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Cancer Research
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3Dオルガノイドによる口腔食道扁平上皮癌のモデリング
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JoVE Journal
Cancer Research
Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Please note that all translations are automatically generated.
Click here for the English version.
3Dオルガノイドによる口腔食道扁平上皮癌のモデリング
DOI:
10.3791/64676-v
•
10:43 min
•
December 23, 2022
•
Samuel Flashner*
1
,
Cecilia Martin*
1,2
,
Norihiro Matsuura
,
Masataka Shimonosono
,
Yasuto Tomita
,
Masaki Morimoto
,
Ogoegbunam Okolo
,
Victoria X. Yu
3
,
Anuraag S. Parikh
3
,
Andres J. P. Klein-Szanto
,
Kelley Yan
2
,
Joel T. Gabre
5
,
Chao Lu
6
,
Fatemeh Momen-Heravi
7
,
Anil K. Rustgi
5
,
Hiroshi Nakagawa
2,5
1
Herbert Irving Comprehensive Cancer Center
,
Columbia University
,
2
Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center
,
Columbia University
,
3
Department of Otolaryngology, Head and Neck Surgery
,
Columbia University
,
4
Histopathology Facility
,
Fox Chase Cancer Center
,
5
Division of Digestive and Liver Diseases, Department of Medicine
,
Columbia University
,
6
Department of Genetics and Development
,
Columbia University
,
7
Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine
,
Columbia University
Chapters
00:04
Introduction
01:28
Animal Dissection and Organ Collection
04:39
Isolation of Keratinocytes from the Dissected Mouse Tissue
06:07
Seeding of the Initial Cell Suspension
06:54
Passaging and Cryopreservation of Murine Esophageal Organoids (MEOs)
07:52
Preparation of Organoids for Paraffin Embedding
08:48
Results: Characterization of the Organoids
10:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは、化学的発がんによって誘発される正常、腫瘍前、および扁平上皮がんの病変を表すマウスの口腔食道3Dオルガノイドを生成および特性評価するための重要なステップについて説明します。
Tags
3D Organoids
Esophageal Squamous Cell Carcinoma
ESCC
Mouse Model
4NQO
Genetic Heterogeneity
Tumorigenesis
Therapeutic Strategies
Animal Dissection
Organ Collection
Paraffin-embedding
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