JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
在 3D 类器官中模拟口腔-食管鳞状细胞癌
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Please note that all translations are automatically generated.
Click here for the English version.
在 3D 类器官中模拟口腔-食管鳞状细胞癌
DOI:
10.3791/64676-v
•
10:43 min
•
December 23, 2022
•
Samuel Flashner*
1
,
Cecilia Martin*
1,2
,
Norihiro Matsuura
,
Masataka Shimonosono
,
Yasuto Tomita
,
Masaki Morimoto
,
Ogoegbunam Okolo
,
Victoria X. Yu
3
,
Anuraag S. Parikh
3
,
Andres J. P. Klein-Szanto
,
Kelley Yan
2
,
Joel T. Gabre
5
,
Chao Lu
6
,
Fatemeh Momen-Heravi
7
,
Anil K. Rustgi
5
,
Hiroshi Nakagawa
2,5
1
Herbert Irving Comprehensive Cancer Center
,
Columbia University
,
2
Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center
,
Columbia University
,
3
Department of Otolaryngology, Head and Neck Surgery
,
Columbia University
,
4
Histopathology Facility
,
Fox Chase Cancer Center
,
5
Division of Digestive and Liver Diseases, Department of Medicine
,
Columbia University
,
6
Department of Genetics and Development
,
Columbia University
,
7
Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine
,
Columbia University
Chapters
00:04
Introduction
01:28
Animal Dissection and Organ Collection
04:39
Isolation of Keratinocytes from the Dissected Mouse Tissue
06:07
Seeding of the Initial Cell Suspension
06:54
Passaging and Cryopreservation of Murine Esophageal Organoids (MEOs)
07:52
Preparation of Organoids for Paraffin Embedding
08:48
Results: Characterization of the Organoids
10:03
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了生成和表征鼠口腔 - 食管3D类器官的关键步骤,这些类器官代表通过化学致癌引起的正常,癌前和鳞状细胞癌病变。
Tags
3D Organoids
Esophageal Squamous Cell Carcinoma
ESCC
Mouse Model
4NQO
Genetic Heterogeneity
Tumorigenesis
Therapeutic Strategies
Animal Dissection
Organ Collection
Paraffin-embedding
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在头颈部鳞状细胞癌的神经浸润模型
档案福尔马林固定,石蜡包埋的人类口咽鳞状细胞癌样品中T细胞亚群的四色荧光免疫组织化学
基于球形的头颈部鳞状细胞癌3D 细胞培养模型的治疗试验
舌细胞外基质的制备与舌鳞癌
体外
重建
小鼠鳞状细胞细胞的肿瘤免疫分析及治疗反应评价
评估癌细胞3D球形培养中的细胞活力和死亡
比较在小鼠肾脏和鸡胆膜上建立的转移性透明细胞肾细胞癌模型
多维共同培养系统模拟肺鳞癌进展
监测三D培养中的癌细胞入侵和T细胞毒性
间变性甲状腺癌和头颈部鳞状细胞癌患者来源异种移植模型的建立与表征
Read Article