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Journal
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Cancer Research
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从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官,无需传代
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
Please note that all translations are automatically generated.
Click here for the English version.
从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官,无需传代
DOI:
10.3791/64626-v
•
08:57 min
•
November 11, 2022
•
Serena L. Cornelius
2
,
Megan M. Colonnetta
3,4,5
,
Katherine E. Lake
2
,
Clayton A. Smith
2
,
Yu-An Zhang
2
,
Evanthia T. Roussos-Torres
,
Sangeetha M. Reddy
2
,
Elizabeth H. Chen
3,4,5
,
Isaac S. Chan
2,3,4
1
Department of Internal Medicine, Division of Hematology and Oncology
,
University of Texas Southwestern Medical Center
,
2
Harold C. Simmons Comprehensive Cancer Center
,
University of Texas Southwestern Medical Center
,
3
Department of Molecular Biology
,
University of Texas Southwestern Medical Center
,
4
Hamon Center for Regenerative Science and Medicine
,
University of Texas Southwestern Medical Center
,
5
Department of Cell Biology
,
University of Texas Southwestern Medical Center
,
6
Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine
,
University of Southern California
Chapters
00:04
Introduction
00:55
Collecting, Digesting Tissue, and Differential Centrifugation
04:11
Embedding Organoids in BECM and Collagen
07:22
Results: Immunofluorescent Imaging of Embedded Organoids
08:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议讨论了通过差异离心从原发性正常和肿瘤乳腺组织产生上皮类器官的方法。此外,还包括用于三维培养以及嵌入类器官的免疫荧光成像的说明。
Tags
Keyword Extraction:Epithelial Organoids
Mouse
Human
Tumor
Normal
Passaging
Differential Centrifugation
Collagen
Matrix
Invasion
Mammary Fat Pad
Inguinal
Thoracic
Collagenase
Benchtop Shaking Incubator
Basal Medium
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