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从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官,无需传代
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
DOI:

08:57 min

November 11, 2022

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Chapters

  • 00:04Introduction
  • 00:55Collecting, Digesting Tissue, and Differential Centrifugation
  • 04:11Embedding Organoids in BECM and Collagen
  • 07:22Results: Immunofluorescent Imaging of Embedded Organoids
  • 08:11Conclusion

Summary

Automatic Translation

该协议讨论了通过差异离心从原发性正常和肿瘤乳腺组织产生上皮类器官的方法。此外,还包括用于三维培养以及嵌入类器官的免疫荧光成像的说明。

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