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Cancer Research
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骨転移異種移植マウスモデルを作成するためのヒト前立腺癌細胞の心臓内注射
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JoVE Journal
Cancer Research
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
骨転移異種移植マウスモデルを作成するためのヒト前立腺癌細胞の心臓内注射
DOI:
10.3791/64589-v
•
06:32 min
•
November 04, 2022
•
Junli Chang*
1,2
,
Xingyuan Sun*
1,2
,
Xiaoping Ma
2
,
Peng Zhao
2
,
Binhao Shi
2
,
Yongjun Wang
2
,
Xianghui Han
3
,
Yanping Yang
2
1
Longhua Hospital
,
Shanghai University of Traditional Chinese Medicine
,
2
Key Laboratory of Theory and Therapy of Muscles and Bones
,
Ministry of Education
,
3
Institute of Chinese Traditional Surgery, Longhua Hospital
,
Shanghai University of Traditional Chinese Medicine
Chapters
00:04
Introduction
01:00
Preparation of Prostate Cancer Cells for Injection
02:25
Surgery for Intra‐Cardiac Injection of the Human Prostate Cancer Cells
05:03
Results: Intra‐Cardiac Injection of Prostate Cancer Cells Induced Metastatic Lesions in Mice
06:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、骨転移病変を有するマウスモデルを生成するためのヒト前立腺癌細胞の心臓内注射のためのプロトコルを提示する。
Tags
Intra-cardiac Injection
Prostate Cancer Cells
Bone Metastasis
Xenograft Mouse Model
Luciferase-labeled PC3 Cells
Cell Culture
Trypsinization
Cell Counting
Centrifugation
Cell Suspension
Anesthesia
Surgical Procedure
Xiphoid Process
Jugular Notch
Intercostal Space
Injection Point
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