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Cancer Research
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心内注射人前列腺癌细胞以创建骨转移异种移植小鼠模型
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Cancer Research
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JoVE Journal
Cancer Research
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
心内注射人前列腺癌细胞以创建骨转移异种移植小鼠模型
DOI:
10.3791/64589-v
•
06:32 min
•
November 04, 2022
•
Junli Chang*
1,2
,
Xingyuan Sun*
1,2
,
Xiaoping Ma
2
,
Peng Zhao
2
,
Binhao Shi
2
,
Yongjun Wang
2
,
Xianghui Han
3
,
Yanping Yang
2
1
Longhua Hospital
,
Shanghai University of Traditional Chinese Medicine
,
2
Key Laboratory of Theory and Therapy of Muscles and Bones
,
Ministry of Education
,
3
Institute of Chinese Traditional Surgery, Longhua Hospital
,
Shanghai University of Traditional Chinese Medicine
Chapters
00:04
Introduction
01:00
Preparation of Prostate Cancer Cells for Injection
02:25
Surgery for Intra‐Cardiac Injection of the Human Prostate Cancer Cells
05:03
Results: Intra‐Cardiac Injection of Prostate Cancer Cells Induced Metastatic Lesions in Mice
06:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种用于心内注射人前列腺癌细胞的方案,以生成具有骨转移病变的小鼠模型。
Tags
Intra-cardiac Injection
Prostate Cancer Cells
Bone Metastasis
Xenograft Mouse Model
Luciferase-labeled PC3 Cells
Cell Culture
Trypsinization
Cell Counting
Centrifugation
Cell Suspension
Anesthesia
Surgical Procedure
Xiphoid Process
Jugular Notch
Intercostal Space
Injection Point
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