Journal
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Propagazione del virus e quantificazione colorimetrica cellulare
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal Immunology and Infection
Virus Propagation and Cell-Based Colorimetric Quantification
DOI:

07:26 min

April 07, 2023

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Chapters

  • 00:00Introduction
  • 00:43Virus Propagation
  • 02:15Virus Quantification
  • 04:31Staining
  • 05:29Results: Foci Forming Assay for Zika Virus P6-740 in 24 and 96-Well Plates
  • 06:55Conclusion

Summary

Automatic Translation

Il presente protocollo descrive la propagazione del virus Zika (ZIKV) in cellule renali di scimmia verde africana Vero e la quantificazione di ZIKV utilizzando metodi di immunorilevazione colorimetrica basati su cellule nei formati a 24 e 96 pozzetti (high throughput).

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