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Journal
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Immunology and Infection
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病毒传播和基于细胞的比色定量
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Virus Propagation and Cell-Based Colorimetric Quantification
Please note that all translations are automatically generated.
Click here for the English version.
病毒传播和基于细胞的比色定量
DOI:
10.3791/64578-v
•
07:26 min
•
April 07, 2023
•
Jia-Yi Tan
,
Jo-Ern Wong
,
Nurhafiza Zainal
,
Sazaly AbuBakar
,
Kim-Kee Tan
1
Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE)
,
Universiti Malaya
,
2
Department of Medical Microbiology, Faculty of Medicine
,
Universiti Malaya
Chapters
00:00
Introduction
00:43
Virus Propagation
02:15
Virus Quantification
04:31
Staining
05:29
Results: Foci Forming Assay for Zika Virus P6-740 in 24 and 96-Well Plates
06:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本方案描述了寨卡病毒(ZIKV)在Vero非洲绿猴肾细胞中的繁殖,以及使用基于细胞的比色免疫检测方法以24孔和96孔(高通量)形式对ZIKV进行定量。
Tags
Virus Propagation
Cell-based Colorimetric Quantification
Zika Virus
Antibody Recognition
Virus Serotypes
High-throughput Applications
Automated Imaging System
Vero Cells
Cell Culture
Virus Inoculum
Virus Quantification
Serial Dilution
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