JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
抗体依存性細胞傷害修飾化合物の同定のためのハイコンテントスクリーニングアッセイ(英語)
JoVE Journal
Cancer Research
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds
Please note that all translations are automatically generated.
Click here for the English version.
抗体依存性細胞傷害修飾化合物の同定のためのハイコンテントスクリーニングアッセイ(英語)
DOI:
10.3791/64485-v
•
13:59 min
•
August 18, 2023
•
Eliza Guti*
1,2
,
Ákos Máté Bede*
1,3
,
Csongor Váróczy*
1,3
,
Csaba Hegedűs
,
Máté Á. Demény
4
,
László Virág
4
1
Department of Medical Chemistry, Faculty of Medicine
,
University of Debrecen
,
2
Doctoral School of Molecular Medicine
,
University of Debrecen
,
3
National Academy of Scientist Education
,
University of Debrecen
,
4
ELKH-DE Cell Biology and Signaling Research Group
Chapters
00:05
Introduction
01:02
Protocol
01:25
Coating of the HCS plate
02:04
Seeding of JIMT-1 EGFP cells
04:33
Pre-treatment of JIMT-1 EGFP cells with compound library
06:06
Starting the ADCC assay by adding the effector cells
09:16
Imaging
10:45
Image analysis
12:24
Representative results
13:15
Discussion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルは、治療用抗HER-2抗体の存在下でナチュラルキラー細胞媒介性乳がん細胞死滅を調節する化合物を同定するための自動化された画像ベースのハイスループット技術を提示します。
Tags
ADCC
Antibody-dependent Cellular Cytotoxicity
NK Cells
Granulocytes
Macrophages
Effector Cells
FcγR Receptors
Trastuzumab
HER2
JIMT-1 Cells
NK-92 Cells
High-content Screening
96-well Plate
Cell Culture
Cell Attachment
Cell Counting
Trypan Blue
Cell Suspension
High-throughput Screening
Robotic Arm
Pin Tool
Test Compounds
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
病変同定のための乳腺全体マウントの分割
流れの Cytometry ベースの医薬品スクリーニング システム神経膠芽腫幹細胞の細胞分化を促進する小さい分子の同定に関する研究
ドットの試金を使用して細胞シートの移行を分析するには
超音波の利用ガイド生物学的関連性の転移性腫瘍の異種移植片の確立のため組織指示細胞注入
GPC3 ターゲット バイスペシフィック抗体、GPC3 S Fab 強力な細胞毒性を持つ
車 T 細胞スフェロイド殺害アッセイ
キメラ抗原受容体(CAR)T細胞の養子免疫療法用
アレイ化されたライブラリの中スループットスクリーニングとデュアルルシファーゼベースレポーターを用いた転写因子レギュレータの同定
直交トピック膵腫瘍の生成と腫瘍浸潤T細胞細胞傷害の
Ex生体内
特性評価
ゼブラフィッシュにおける原発性腫瘍異種移植片の薬物スクリーニング
Read Article