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Cancer Research
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用于鉴定抗体依赖性细胞毒性修饰化合物的高内涵筛选检测
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Cancer Research
High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds
Please note that all translations are automatically generated.
Click here for the English version.
用于鉴定抗体依赖性细胞毒性修饰化合物的高内涵筛选检测
DOI:
10.3791/64485-v
•
13:59 min
•
August 18, 2023
•
Eliza Guti*
1,2
,
Ákos Máté Bede*
1,3
,
Csongor Váróczy*
1,3
,
Csaba Hegedűs
,
Máté Á. Demény
4
,
László Virág
4
1
Department of Medical Chemistry, Faculty of Medicine
,
University of Debrecen
,
2
Doctoral School of Molecular Medicine
,
University of Debrecen
,
3
National Academy of Scientist Education
,
University of Debrecen
,
4
ELKH-DE Cell Biology and Signaling Research Group
Chapters
00:05
Introduction
01:02
Protocol
01:25
Coating of the HCS plate
02:04
Seeding of JIMT-1 EGFP cells
04:33
Pre-treatment of JIMT-1 EGFP cells with compound library
06:06
Starting the ADCC assay by adding the effector cells
09:16
Imaging
10:45
Image analysis
12:24
Representative results
13:15
Discussion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了一种自动化的、基于图像的高通量技术,用于在存在治疗性抗 HER-2 抗体的情况下鉴定调节自然杀伤细胞介导的乳腺癌细胞杀伤的化合物。
Tags
ADCC
Antibody-dependent Cellular Cytotoxicity
NK Cells
Granulocytes
Macrophages
Effector Cells
FcγR Receptors
Trastuzumab
HER2
JIMT-1 Cells
NK-92 Cells
High-content Screening
96-well Plate
Cell Culture
Cell Attachment
Cell Counting
Trypan Blue
Cell Suspension
High-throughput Screening
Robotic Arm
Pin Tool
Test Compounds
Article
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