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用于生物医学应用的荧光寿命宏观成像仪

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Biology

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JoVE Journal Biology

Fluorescence Lifetime Macro Imager for Biomedical Applications

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用于生物医学应用的荧光寿命宏观成像仪

DOI:

10.3791/64321-v

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06:01 min

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April 07, 2023

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Rajannya Sen, Alexander V. Zhdanov, Ciaran Devoy, Mark Tangney, Liisa M. Hirvonen, Andrei Nomerotski, Dmitri B. Papkovsky

¹School of Biochemistry and Cell Biology,University College Cork, ²Cancer Research@UCC,University College Cork, ³Centre for Microscopy, Characterisation and Analysis (CMCA),The University of Western Australia, ⁴Physics Department,Brookhaven National Laboratory

Chapters

00:04Introduction
00:52PLIM Imaging Setup
02:35Image Acquisition
04:06Data Analysis
04:59Results: Imaging of Phosphorescent Solid-State Sensor
05:19Conclusion

Summary

Automatic Translation

本文描述了一种新型快速光学成像仪在长衰变发射样品的宏观光致发光寿命成像中的应用。描述了集成、图像采集和分析程序，以及用于成像的传感器材料的制备和表征以及成像仪在研究生物样品中的应用。

Tags

Fluorescence Lifetime Macro Imager Biomedical Applications Phosphorescence Lifetime Oxygen Concentration TCSPC Cricket Adapter Photonis PP0360EF Intensifier 650 Nm Emission Filter TPX Three-cam Camera Module 390 Nm LED SOFI Software Medipix/Timepix Frames Time Over Threshold

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