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Fluorescence Lifetime Macro Imager for Biomedical Applications
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JoVE Journal Biology
Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

DOI:
10.3791/64321-v

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06:01 min

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April 07, 2023

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, , , , , ,
1School of Biochemistry and Cell Biology,University College Cork, 2Cancer Research@UCC,University College Cork, 3Centre for Microscopy, Characterisation and Analysis (CMCA),The University of Western Australia, 4Physics Department,Brookhaven National Laboratory

Chapters

  • 00:04Introduction
  • 00:52PLIM Imaging Setup
  • 02:35Image Acquisition
  • 04:06Data Analysis
  • 04:59Results: Imaging of Phosphorescent Solid-State Sensor
  • 05:19Conclusion

Summary

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This paper describes the use of a new, fast optical imager for the macroscopic photoluminescence lifetime imaging of long decay emitting samples. The integration, image acquisition, and analysis procedures are described, along with the preparation and characterization of the sensor materials for the imaging and the application of the imager in studying biological samples.

Tags

Fluorescence LifetimeMacro ImagerBiomedical ApplicationsPhosphorescence LifetimeOxygen ConcentrationTCSPCCricket AdapterPhotonis PP0360EF Intensifier650 Nm Emission FilterTPX Three-cam Camera Module390 Nm LEDSOFI SoftwareMedipix/Timepix FramesTime Over Threshold

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