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基于声流体微器件的细胞和细胞核可压缩性测量
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Cancer Research
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JoVE Journal Cancer Research
Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
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基于声流体微器件的细胞和细胞核可压缩性测量

DOI:
10.3791/64225-v

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09:06 min

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July 14, 2022

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, , ,
1Sino-French Institute of Nuclear Engineering and Technology,Sun Yat-sen University

Chapters

  • 00:05Introduction
  • 00:46Fabricating and Assembly of the Acoustofluidic Microdevice
  • 03:02Sample Preparation
  • 05:41Measuring the Compressibility of Cell and Nucleus
  • 07:29Results: Measuring Cell or Nucleus Compressibility Based on Acoustofluidic Microdevice
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

这里提出了一个协议，用于构建一个基于声流体微器件的快速且无损的系统来测量细胞或细胞核的可压缩性。考察了上皮-间充质转化或电离辐射后肿瘤细胞力学性能的变化，证明了该方法在科学研究和临床上的应用前景。

Tags

Cell MechanicsTumor MetastasisMalignant TransformationRadiosensitivityAcoustofluidic MethodSuspended StatesEpithelial To Mesenchymal TransitionCirculating Tumor CellsCancer DiagnosisPDMSPlasma CleanerPTFE CatheterStainless Steel NeedlePiezoelectric CeramicCyanoacrylate GluePolystyrene Standard Particle SolutionsCell Suspensions

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