Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Madison I. Moss; Mariela Pauli; Joshua M. Moreau; Jarish N. Cohen; Michael D. Rosenblum; Margaret M. Lowe

doi:10.3791/64040

Journal

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Immunology and Infection

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异种移植皮肤模型在体内操纵人类免疫反应

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Immunology and Infection
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JoVE Journal Immunology and Infection

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Please note that all translations are automatically generated. Click here for the English version.

异种移植皮肤模型在体内操纵人类免疫反应

DOI:

10.3791/64040-v

•

08:23 min

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June 29, 2022

•

Madison I. Moss, Mariela Pauli, Joshua M. Moreau, Jarish N. Cohen, Michael D. Rosenblum, Margaret M. Lowe

¹Department of Dermatology,UCSF, ²TRex Bio

Chapters

00:04Introduction
00:59Processing of Donor Human Skin Sample
02:15Pre-Surgery Conditioning and Preparation
03:42Transplantation Procedure
06:09Results: Analysis of the Xenografts
07:35Conclusion

Summary

Automatic Translation

本协议描述了如何将人体皮肤移植到非肥胖糖尿病（NOD）-scid白细胞介素-2γ链受体（NSG）小鼠上。报告中详细介绍了移植用人体皮肤的制备、移植用小鼠的准备、裂层人体皮肤的移植以及移植后的恢复程序。

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