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Immunology and Infection
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Xenograft Skin Model to Manipulate Human Immune Responses In Vivo
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Immunology and Infection
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JoVE Journal Immunology and Infection
Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

DOI:
10.3791/64040-v

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08:23 min

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June 29, 2022

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, , , , ,
1Department of Dermatology,UCSF, 2TRex Bio

Chapters

  • 00:04Introduction
  • 00:59Processing of Donor Human Skin Sample
  • 02:15Pre-Surgery Conditioning and Preparation
  • 03:42Transplantation Procedure
  • 06:09Results: Analysis of the Xenografts
  • 07:35Conclusion

Summary

Automatic Translation

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The present protocol describes how to graft human skin onto non-obese diabetic (NOD)-scid interleukin-2 gamma chain receptor (NSG) mice. A detailed description of the preparation of human skin for transplant, preparation of mice for transplant, transplantation of split-thickness human skin, and post-transplantation recovery procedure are included in the report.

Tags

Xenograft Skin ModelHuman Immune ResponseIn VivoSkin TransplantSkin InflammationDermatomeSkin GraftingMouse ModelAnti-Gr1Hair Removal

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