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使用福尔马林固定和石蜡包埋组织样品的质谱成像扩展肿瘤微环境的理解
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Cancer Research
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JoVE Journal
Cancer Research
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
Please note that all translations are automatically generated.
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使用福尔马林固定和石蜡包埋组织样品的质谱成像扩展肿瘤微环境的理解
DOI:
10.3791/64015-v
•
06:47 min
•
June 29, 2022
•
Leticia Campos Clemente
,
Ou Shi
,
Frank Rojas
,
Edwin Roger Parra
1
Department of Translational Molecular Pathology
,
The University of Texas MD Anderson Cancer Center
Chapters
00:04
Introduction
01:06
Antibody Panel Preparation and Antibody Staining
02:35
Image Acquisition
04:31
Results: Isotopic Purity and Channel Cross-Talk, Staining Images in Different Tissues, and Images of the Staining Before and After Image Preparation
05:59
Conclusion
Summary
Automatic Translation
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在癌症免疫治疗时代,人们对阐明肿瘤微环境动力学的兴趣惊人地增加。该协议详细介绍了质谱成像技术及其染色和成像步骤,允许高度多路复用的空间分析。
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Mass Spectrometry Imaging
Immune Cell Characterization
40-marker Panel
Antibody Optimization
Single Cell Resolution
Marker Co-expression
Spatial Analysis
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