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一种报告基因检测法,用于分析哺乳动物细胞中内旋微RNA成熟
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
DOI:

06:48 min

June 16, 2022

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Chapters

  • 00:04Introduction
  • 00:52Cell Culture and HuR Overexpression
  • 02:26The Reporter Assay
  • 05:18Results: MicroRNA Biogenesis Reporter Assay Analyzing Maturation in Mammalian Cells
  • 06:17Conclusion

Summary

Automatic Translation

我们开发了一种内旋microRNA生物发生报告基因检测方法,用于具有四种质粒 的体外 细胞:一种具有内旋性miRNA,一种具有靶标,一种用于过表达调节蛋白,另一种用于Renilla荧光素酶。miRNA经过处理,可以通过与靶序列结合来控制荧光素酶的表达。

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