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Journal
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Cancer Research
/
原発性悪性胸膜中皮腫腫瘍ラインの生成と拡張
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Please note that all translations are automatically generated.
Click here for the English version.
原発性悪性胸膜中皮腫腫瘍ラインの生成と拡張
DOI:
10.3791/63374-v
•
08:01 min
•
April 21, 2022
•
Tamara M. Griffiths
,
Carlos Ramos
,
Cara L. Haymaker
1
Department of Translational Molecular Pathology
,
University of Texas MD Anderson Cancer Center
Chapters
00:05
Introduction
00:45
Digestion of Tumor Tissue
03:16
Generation of Primary Tumor Cell Line
04:47
Characterization and Banking of Established Primary Tumor Cell Line
06:13
Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
07:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
この方法論文の目標は、外科的に切除された胸膜中皮腫からの原発性腫瘍細胞株の濃縮、生成、および拡大のための堅牢で再現可能な方法論を実証することである。
Tags
Primary Malignant Pleural Mesothelioma
Tumor Cell Expansion
In Vitro Model
Tumor Digesting Enzymatic Cocktail
Tumor Dissociation
Cell Strainer
Tumor Digestion Medium
Cell Culture
Fibroblast Contamination
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