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Cancer Research
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食道腺癌オルガノイドの継代培養と凍結保存:単一細胞消化の長所と短所
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion
Please note that all translations are automatically generated.
Click here for the English version.
食道腺癌オルガノイドの継代培養と凍結保存:単一細胞消化の長所と短所
DOI:
10.3791/63281-v
•
10:42 min
•
July 06, 2022
•
Ningbo Fan*
1
,
Lisa Raatz*
1
,
Seung-Hun Chon
,
Alexander Quaas
,
Christiane Bruns
,
Yue Zhao
1
Department of General, Visceral, Cancer and Transplantation Surgery
,
University Hospital Cologne
,
2
Institute of Pathology
,
University Hospital Cologne
Chapters
00:05
Introduction
00:53
Preparations in Advance
01:23
Harvesting Organoids
03:05
Subculturing without Digestion
04:23
Subculturing with Single-Cell Digestion
06:48
Cryopreservation of the Undigested and Single Cell Digested PDOs
08:08
Results: Observation and Identification of Cell Culture
09:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、単一細胞消化の有無にかかわらず食道腺癌オルガノイドの継代培養および凍結保存の方法を記述し、研究者が実験計画に基づいて適切な戦略を選択できるようにする。
Tags
Esophageal Adenocarcinoma Organoids
Subculture
Single-cell Digestion
PDOs
ECM Gel
Cell Recovery Solution
Centrifugation
Cryopreservation
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