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食管腺癌类器官的传代培养和冷冻保存：单细胞消化的利弊
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion
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食管腺癌类器官的传代培养和冷冻保存：单细胞消化的利弊

DOI:
10.3791/63281-v

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10:42 min

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July 06, 2022

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, , , , ,
1Department of General, Visceral, Cancer and Transplantation Surgery,University Hospital Cologne, 2Institute of Pathology,University Hospital Cologne

Chapters

  • 00:05Introduction
  • 00:53Preparations in Advance
  • 01:23Harvesting Organoids
  • 03:05Subculturing without Digestion
  • 04:23Subculturing with Single-Cell Digestion
  • 06:48Cryopreservation of the Undigested and Single Cell Digested PDOs
  • 08:08Results: Observation and Identification of Cell Culture
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了食管腺癌类器官的传代培养和冷冻保存方法，有和没有单细胞消化，使研究人员能够根据其实验设计选择适当的策略。

Tags

Esophageal Adenocarcinoma OrganoidsSubcultureSingle-cell DigestionPDOsECM GelCell Recovery SolutionCentrifugationCryopreservation

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