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SNARE人身売買を用いた循環型の内視鏡分析法
JoVE Journal
Biology
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JoVE Journal Biology
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
DOI:

08:51 min

February 12, 2022

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Chapters

  • 00:06Introduction
  • 01:04Seeding of Mouse Melanocytes on Pre-Treated Coverslips
  • 01:42Transfection of Cells with the STX13 Plasmids
  • 02:39Fixation of the Cells
  • 03:11Immunostaining of the Cells
  • 05:05Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
  • 07:25Results: Quantification of Recycling Endosomes’ Tublar Number and Length
  • 07:56Conclusion

Summary

Automatic Translation

子宮内膜のリサイクルは、内膜管状ネットワークの一部です。ここでは、GFP-STX13をオルガネラマーカーとして使用して、エンドーソームのリサイクルのダイナミクスを定量化する方法を紹介します。

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