JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
SNARE人身売買を用いた循環型の内視鏡分析法
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
Please note that all translations are automatically generated.
Click here for the English version.
SNARE人身売買を用いた循環型の内視鏡分析法
DOI:
10.3791/63087-v
•
08:51 min
•
February 12, 2022
•
Anshul Milap Bhatt
,
Subba Rao Gangi Setty
1
Department of Microbiology and Cell Biology
,
Indian Institute of Science, Bangalore KA 560012, India
Chapters
00:06
Introduction
01:04
Seeding of Mouse Melanocytes on Pre-Treated Coverslips
01:42
Transfection of Cells with the STX13 Plasmids
02:39
Fixation of the Cells
03:11
Immunostaining of the Cells
05:05
Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
07:25
Results: Quantification of Recycling Endosomes’ Tublar Number and Length
07:56
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
子宮内膜のリサイクルは、内膜管状ネットワークの一部です。ここでは、GFP-STX13をオルガネラマーカーとして使用して、エンドーソームのリサイクルのダイナミクスを定量化する方法を紹介します。
Tags
Microscopy-based Assay
Recycling Endosomes
SNARE Trafficking
Melanocytes
Melanosomes
Lysozome Related Organelles (LRO)
Syntaxin-13
Transfection
Basement Membrane Matrix
PMA
OPTI-MEM
RPMI Medium
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
トカゲの運動の速度と方向の視覚感度のテスト
コンピュータによって生成された動物モデルの刺激
ゼブラフィッシュの視覚機能を研究するために視運動性応答を使用して
タンパク質 - タンパク質相互作用を研究するために磁気ビーズを用いて液相アフィニティキャプチャーアッセイ:ポリオ-nanobodyの例
酵素による細胞融合アッセイを使用して、SNARE媒介膜融合の解析
Using
Caenorhabditis elegans
as a Model System to Study Protein Homeostasis in a Multicellular Organism
セルベースのL-グルタチオン保護アッセイ細胞膜タンパク質のエンドサイトーシスとリサイクルを研究するために、
遺伝子発現およびゲノム変化を特徴づけるために、RNAシーケンシングアッセイをターゲットに
SCAPの分析<em> N</em>ヒト細胞における-glycosylationと人身売買
チミジン類似体BrdUを用いたラットの細胞増殖と神経新生を解析するための免疫組織化学技術
Read Article