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基于显微镜的测定,用于使用SNARE运输研究和分析回收内体
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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
Please note that all translations are automatically generated.
Click here for the English version.
基于显微镜的测定,用于使用SNARE运输研究和分析回收内体
DOI:
10.3791/63087-v
•
08:51 min
•
February 12, 2022
•
Anshul Milap Bhatt
,
Subba Rao Gangi Setty
1
Department of Microbiology and Cell Biology
,
Indian Institute of Science, Bangalore KA 560012, India
Chapters
00:06
Introduction
01:04
Seeding of Mouse Melanocytes on Pre-Treated Coverslips
01:42
Transfection of Cells with the STX13 Plasmids
02:39
Fixation of the Cells
03:11
Immunostaining of the Cells
05:05
Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
07:25
Results: Quantification of Recycling Endosomes’ Tublar Number and Length
07:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
回收内体是内体管网络的一部分。在这里,我们提出了一种使用GFP-STX13作为细胞器标记来量化回收内体动力学的方法。
Tags
Microscopy-based Assay
Recycling Endosomes
SNARE Trafficking
Melanocytes
Melanosomes
Lysozome Related Organelles (LRO)
Syntaxin-13
Transfection
Basement Membrane Matrix
PMA
OPTI-MEM
RPMI Medium
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