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荧光显微镜用于人肿瘤细胞和肿瘤异种移植小鼠中巨细胞增多介导的ATP内化
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
DOI:

08:31 min

June 30, 2021

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Chapters

  • 00:05Introduction
  • 01:05Tumor Cell Culture and Xenograft Tumor Development
  • 02:41HMWFD and NHF-ATP Solution Preparation
  • 03:21ATP Internalization in Tumor Xenograft
  • 03:53Cryo-embedding
  • 04:45Tissue Slide Fixation
  • 05:25Fluorescence Microscopy and Image Acquisition
  • 07:12Results: Assessment of Nonhydrolyzable Fluorescent ATP Internalization
  • 07:44Conclusion

Summary

Automatic Translation

我们开发了一种可重复的方法,以可视化具有高细胞分辨率的不可水解荧光三磷酸腺苷(ATP)的内化,ATP替代物。我们使用独立的 体外体内 测定验证了我们的方法 - 人肿瘤细胞系和用人类肿瘤组织异种移植的免疫缺陷小鼠。

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