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从人骨关节炎膝关节收集和RNA提取
JoVE Journal
Biology
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JoVE Journal
Biology
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
Please note that all translations are automatically generated.
Click here for the English version.
从人骨关节炎膝关节收集和RNA提取
DOI:
10.3791/62718-v
•
06:06 min
•
July 22, 2021
•
Thomas Wilson
,
Navdeep Kaur
,
Jason Davis
,
Shabana Amanda Ali
3
1
Bone and Joint Center
,
Henry Ford Health System
,
2
Department of Orthopedic Surgery
,
Henry Ford Health System
,
3
Center for Molecular Medicine and Genetics
,
Wayne State University
Chapters
00:05
Introduction
00:56
Sample Processing
01:54
Hard Tissue Homogenization
03:05
Soft Tissue Homogenization
04:20
Results: Tissue Identification and RNA Quality Assessment
05:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
从全膝关节置换术后的患者获得的原代组织为骨关节炎研究提供了一个实验模型,具有最大的临床可转化性。该协议描述了如何从七个独特的膝关节组织中鉴定,处理和分离RNA,以支持人类骨关节炎的机制研究。
Tags
RNA Extraction
Osteoarthritis
Human Knee Joint
Gene Expression
Histological Analysis
Cartilage
Bone
Meniscus
Infrapatellar Fat Pad
Anterior Cruciate Ligament
Synovium
Vastus Medialis Oblique Muscle
Liquid Nitrogen
Homogenization
Mortar And Pestle
Acid Guanidinium Phenol Solution
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