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Journal
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Immunology and Infection
/
通过荧光
原位
杂交、凝集素染色和成像可视化肠道微生物群与宿主的相互作用
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Visualization of Gut Microbiota-host Interactions via Fluorescence
In Situ
Hybridization, Lectin Staining, and Imaging
Please note that all translations are automatically generated.
Click here for the English version.
通过荧光
原位
杂交、凝集素染色和成像可视化肠道微生物群与宿主的相互作用
DOI:
10.3791/62646-v
•
09:31 min
•
July 09, 2021
•
Katharine M. Ng
2
,
Carolina Tropini
2,3
1
School of Biomedical Engineering
,
University of British Columbia
,
2
Department of Microbiology and Immunology
,
University of British Columbia
,
3
Humans and the Microbiome Program
,
Canadian Institute for Advanced Research (CIFAR)
Chapters
00:04
Introduction
00:56
Tissue Sample Collection and Fixation
02:04
Paraffin Infiltration and Embedding
02:59
Preparation of Slides for Staining and Host Features
04:31
Bacterial Staining with FISH
06:41
Results: Analysis of the Localization of
Muribaculum intestinale
Relative to Mucus in a Gnotobiotic Mouse Model and Some Common Imaging Problems
08:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种简化的实验方案详细介绍了检测和成像复杂组织样品中细菌的工作流程,从固定组织到用荧光
原位
杂交染色微生物。
Tags
Gut Microbiota
Host-associated Microbes
Fluorescence In Situ Hybridization (FISH)
Lectin Staining
Imaging
Host-microbiome Interface
Methacarn Fixation
Paraffin Embedding
Intestinal Sections
Visualization
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