A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Surgery,University Medical Center Schleswig-Holstein, 2Institute of Medical and Marine Biotechnology,University of Lübeck, 3Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, 4Institute for Surgical Pathology, Medical Center – University of Freiburg, Faculty of Medicine,University of Freiburg, 5Tumorbank Comprehensive Cancer Center Freiburg,Medical Center – University of Freiburg, 6Core Facility Histopathology and Digital Pathology Freiburg,Medical Center – University of Freiburg
Chapters
- 00:00Introduction
- 00:54Preparation of Low-Melting Agarose and Vibratome
- 01:58Tissue Embedding in Low-Melting Agarose
- 03:13Slicing of the Agarose-Embedded Tissue Using a Vibratome
- 03:51Culture of Organotypic Slice Cultures
- 04:38Formalin Fixation and Paraffin Embedding of OTSCs
- 06:20Hematoxylin and Eosin Staining
- 07:09Results: Morphology, Viability, and Histopathological Evaluation of the OTSCs at 6 and 15 Days of Primary PDAC and Metastasis
- 08:50Conclusion
