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1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine,Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine,Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
Chapters
- 00:01Introduction
- 00:47Murine Hepatocyte Isolation by Liver Perfusion and Digestion
- 02:47Hepatocyte Organoids Culture
- 04:29Single Cells from Hepatocyte Organoids
- 07:22siRNA or Plasmid Transfection
- 12:58Representative Results
- 13:58Conclusion
Se estableció un sistema de cultivo de organoides 3D ex vitro a largo plazo a partir de hepatocitos de ratón. Estos organoides pueden ser atravesados y manipulados genéticamente por infección por lentivirus de construcción shRNA/ectópica, transfección de siRNA e ingeniería CRISPR-Cas9.
