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Journal
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Biology
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小鼠肝细胞类器官的建立与遗传操作
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Biology
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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
Please note that all translations are automatically generated.
Click here for the English version.
小鼠肝细胞类器官的建立与遗传操作
DOI:
10.3791/62438-v
•
14:54 min
•
February 12, 2022
•
Jiabei Lian*
1,2
,
Xinyuan Meng*
1,3
,
Xiaohui Zhang
2
,
Huili Hu
2
1
Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine
,
Shandong University
,
2
The Research Center of Stem Cell and Regenerative Medicine
,
Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
Chapters
00:01
Introduction
00:47
Murine Hepatocyte Isolation by Liver Perfusion and Digestion
02:47
Hepatocyte Organoids Culture
04:29
Single Cells from Hepatocyte Organoids
07:22
siRNA or Plasmid Transfection
12:58
Representative Results
13:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
从小鼠肝细胞建立了长期
的体外
3D类器官培养系统。这些类器官可以通过shRNA/异位构建的慢病毒感染,siRNA转染和CRISPR-Cas9工程进行遗传和遗传操纵。
Tags
Hepatocyte
Organoids
Liver
Metabolism
Detoxification
Regeneration
In Vitro Culture
Genetic Modification
Liver Perfusion
Liver Digestion
Single Cell Suspension
Organoid Culture
Metrogel
Passage
Genetic Manipulation
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