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小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
DOI:

08:43 min

May 07, 2021

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Chapters

  • 00:05Introduction
  • 00:58Microdissection of SAN and AVN
  • 03:14Digestion
  • 04:02Magnetic Enrichment of CD45 and Sample Staining
  • 05:28Cell Sorting and Gating Strategy
  • 06:10Results: Identification of SAN and AVN and Analysis of Sorted Cardiac Macrophages
  • 08:17Conclusion

Summary

Automatic Translation

这里介绍的方案提供了一种从小鼠心脏的窦房结(SAN)和房室结(AVN)区域分离心脏驻留巨噬细胞的分步方法。

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