JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
Please note that all translations are automatically generated.
Click here for the English version.
小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养
DOI:
10.3791/62236-v
•
08:43 min
•
May 07, 2021
•
Ruibing Xia
2,3
,
Simone Loy
,
Stefan Kääb
3
,
Anna Titova
,
Christian Schulz
2,3
,
Steffen Massberg
2,3
,
Sebastian Clauss
2,3
1
University Hospital Munich, Department of Medicine I
,
Ludwig-Maximilian-Unversity Munich (LMU)
,
2
Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich
,
Ludwig-Maximilians-University (LMU)
,
3
German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)
Chapters
00:05
Introduction
00:58
Microdissection of SAN and AVN
03:14
Digestion
04:02
Magnetic Enrichment of CD45 and Sample Staining
05:28
Cell Sorting and Gating Strategy
06:10
Results: Identification of SAN and AVN and Analysis of Sorted Cardiac Macrophages
08:17
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这里介绍的方案提供了一种从小鼠心脏的窦房结(SAN)和房室结(AVN)区域分离心脏驻留巨噬细胞的分步方法。
Tags
Cardiac Macrophages
Sinus Node
Atrioventricular Node
Microdissection
Fluorescence-activated Cell Sorting
Cardiac Electrophysiology
Mouse
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
小鼠胰岛的分离和包膜下肾移植的方法
来自患者的细胞培养和分离的CD133<sup> +</sup>假定的肿瘤干细胞的恶性黑色素瘤
分离,冻存和文化的人羊膜上皮细胞的临床应用
可视化<em>肺炎链球菌</em>在心脏Microlesions和随后的心脏重构
从小鼠癌症模型的循环肿瘤细胞的分离和繁殖
分离培养犬从动脉和静脉主血管内皮细胞
鼠心围细胞的分离与纯化
从成年小鼠分离心膜肌细胞
单肌纤维隔离与文化从埃默里-德雷福斯肌肉萎缩的早期后纳塔尔发展穆林模型
穆林·斯特拉特·甘利翁的位置、解剖和分析
Read Article