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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

DOI:
10.3791/62236-v

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08:43 min

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May 07, 2021

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, , , , , ,
1University Hospital Munich, Department of Medicine I,Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich,Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

Chapters

  • 00:05Introduction
  • 00:58Microdissection of SAN and AVN
  • 03:14Digestion
  • 04:02Magnetic Enrichment of CD45 and Sample Staining
  • 05:28Cell Sorting and Gating Strategy
  • 06:10Results: Identification of SAN and AVN and Analysis of Sorted Cardiac Macrophages
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

Tags

Cardiac MacrophagesSinus NodeAtrioventricular NodeMicrodissectionFluorescence-activated Cell SortingCardiac ElectrophysiologyMouse

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