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Immunology and Infection
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使用来自同一物种的抗体进行双标记免疫荧光研究宿主 - 病原体相互作用
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
Please note that all translations are automatically generated.
Click here for the English version.
使用来自同一物种的抗体进行双标记免疫荧光研究宿主 - 病原体相互作用
DOI:
10.3791/62219-v
•
07:35 min
•
July 10, 2021
•
Camila Gachet-Castro*
1
,
Lays Adrianne Mendonça Trajano-Silva*
1
,
Munira Muhammad Abdel Baqui
1
Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School
,
University of Sao Paulo, Ribeirao Preto, SP, Brazil
Chapters
00:04
Introduction
01:21
Parasite Cultures
02:00
Control Immunofluorescence Protocol
03:23
Double Labeling Immunofluorescence ProtocolUsing Monoclonal and Polyclonal Antibodies Raised in the Same Host
04:18
Triple Labeling Immunofluorescence Protocol with the Addition of the Third Polyclonal Antibody from Different Host Species
05:16
Confocal Imaging Acquisition
05:39
Results: Host-pathogen Interactions Studies Using Double Labeling Immunofluorescence
06:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,该协议描述了如何使用在同一物种中培养的一抗来研究宿主 - 病原体相互作用来执行双重标记免疫荧光。此外,它可以包括来自该协议中不同宿主的第三种抗体。这种方法可以在任何细胞类型和病原体中进行。
Tags
Double Labeling
Immunofluorescence
Antibodies
Same Species
Host-pathogen Interactions
Trypanosoma Cruzi
LLC-MK2 Cells
Polyclonal Antibody
Monoclonal Antibody
Phalloidin
Actin Filaments
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