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无标签定量蛋白经济学工作流程,用于发现驱动的宿主-病原体交互
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
DOI:

05:37 min

October 20, 2020

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Chapters

  • 00:05Introduction
  • 01:14Macrophage Infection
  • 02:07Infection Verification
  • 03:04Sample Collection
  • 03:47Results: Representative Effects of C. neoformans Macrophage Infection
  • 05:04Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议,以分析宿主和病原体之间的相互作用,在感染基于质谱学的蛋白组学。此协议使用无标签量化来测量宿主(例如巨噬细胞)和病原体(例如 新福尔曼隐球菌)的蛋白质丰度变化。

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