JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
无标签定量蛋白经济学工作流程,用于发现驱动的宿主-病原体交互
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Please note that all translations are automatically generated.
Click here for the English version.
无标签定量蛋白经济学工作流程,用于发现驱动的宿主-病原体交互
DOI:
10.3791/61881-v
•
05:37 min
•
October 20, 2020
•
Brianna Ball
,
Arjun Sukumaran
,
Jennifer Geddes-McAlister
1
Molecular and Cellular Biology Department
,
University of Guelph
Chapters
00:05
Introduction
01:14
Macrophage Infection
02:07
Infection Verification
03:04
Sample Collection
03:47
Results: Representative Effects of
C. neoformans
Macrophage Infection
05:04
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提出了一个协议,以分析宿主和病原体之间的相互作用,在感染基于质谱学的蛋白组学。此协议使用无标签量化来测量宿主(例如巨噬细胞)和病原体(例如
新福尔曼隐球菌)的
蛋白质丰度变化。
Tags
Label-free
Quantitative Proteomics
Host-pathogen Interactions
Fungal Infection
Macrophage Response
Cryptococcus
Anti-virulent Strategies
Bioinformatics Pipeline
LDH Assay
Cytotoxicity
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
动态活细胞成像的宿主 - 病原体相互作用的研究使用的光学陷阱
使用的EpiAirway模型,用于描述长期宿主 - 病原体相互作用
比较方法来表征景观的宿主 - 病原体蛋白 - 蛋白相互作用
在乳腺癌检测人类白细胞抗原生物标志物的利用无标记生物传感技术
电功能性细菌效应器到哺乳动物细胞
RNA的疗效和毒性的评价基因或药物治疗靶向HIV-1的生产使用
一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜
体内
人淋巴组织和女性生殖器粘膜与人体免疫机能丧失病毒1和 Histoculture 的感染
闭环惯性微流体在患者源气道分泌物中无标签中性粒细胞富集的应用
用三维定量相位成像和机器学习技术对淋巴细胞亚型进行无标签鉴定
Read Article