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卵巢组织细菌的特征和功能预测
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Cancer Research
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JoVE Journal Cancer Research
Characterization and Functional Prediction of Bacteria in Ovarian Tissues
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卵巢组织细菌的特征和功能预测

DOI:
10.3791/61878-v

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10:12 min

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October 23, 2021

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, , , , , , , , , , , ,
1Department of Obstetrics and Gynecology,First Affiliated Hospital of Xi’an Jiaotong University, 2School of Social Sciences,University of Manchester, 3Department of Pathology,First Affiliated Hospital of Xi’an Jiaotong University, 4Omega Bioservices Inc

Chapters

  • 00:03Introduction
  • 01:10Collect Samples
  • 01:47Perform Bacterial LPS’s: Immunohistochemistry in Ovaries
  • 03:50Detect 16S rRNA Sequencing
  • 05:0216S rRNA Sequencing Analysis
  • 06:09Predict Bacteria Function
  • 06:36Data
  • 07:09Representative Result
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

为了发现和区分原位癌和非癌卵巢组织的细菌，进行了免疫造血染色和16S核糖核化核糖核素（16S rRNA基因）测序。通过利用"未观测状态"（PICRUST）重建对社区的虫基和邻苯二甲酸酯遗传学调查来预测细菌的组成和功能差异。

Tags

BacteriaImmunohistochemistry16S RRNA SequencingCharacterizationFunctional PredictionCancerNon-cancerBoPsCellular-genetic InvestigationIn SituParaffin EmbeddingAntigen RetrievalAntibody StainingDAP SubstrateHRP PolymerDehydration16S RRNA Sequencing Library Preparation

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