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Decellularisatie van het Murine Cardiopulmonaal Complex
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Decellularization of the Murine Cardiopulmonary Complex
DOI:

08:34 min

May 30, 2021

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Chapters

  • 00:04Introduction
  • 00:50Post-Mortem Microsurgery
  • 03:26Decellularization and Immunostaining
  • 05:30Hematoxylin-eosin Staining
  • 07:08Results: Multiple Protein Immunostaining of Decellularized Mouse Lungs
  • 07:48Conclusion

Summary

Automatic Translation

Dit protocol heeft tot doel het hart en de longen van muizen te decellulariseren. De resulterende extracellulaire matrix (ECM) steigers kunnen immunos gekleurd en afgebeeld worden om de locatie en topologie van hun componenten in kaart te brengen.

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