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鼠心肺复合物的脱细胞化
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Cancer Research
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JoVE Journal Cancer Research
Decellularization of the Murine Cardiopulmonary Complex
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鼠心肺复合物的脱细胞化

DOI:
10.3791/61854-v

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08:34 min

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May 30, 2021

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, , , , ,
1Biotech Research and Innovation Centre (BRIC),University of Copenhagen (UCPH), 2Department of Laboratory Medicine, Division of Translational Cancer Research,Lund University

Chapters

  • 00:04Introduction
  • 00:50Post-Mortem Microsurgery
  • 03:26Decellularization and Immunostaining
  • 05:30Hematoxylin-eosin Staining
  • 07:08Results: Multiple Protein Immunostaining of Decellularized Mouse Lungs
  • 07:48Conclusion

Summary

Automatic Translation

Automatic Translation

该协议旨在使小鼠的心脏和肺部脱细胞。可以对所得的细胞外基质（ECM）支架进行免疫染色和成像，以绘制其组分的位置和拓扑结构。

Tags

DecellularizationMurineCardiopulmonary ComplexExtracellular MatrixStructural IntegrityBiochemical AnalysisAnatomical AnalysisThoracic WallSternotomyThymusBrachiocephalic ArteriesTracheaBronchiDescending Aorta

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