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使用 COMSTAT 软件量化抗菌剂对体外生物膜结构的影响

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Immunology and Infection

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JoVE Journal Immunology and Infection

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

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使用 COMSTAT 软件量化抗菌剂对体外生物膜结构的影响

DOI:

10.3791/61759-v

•

06:18 min

•

December 14, 2020

•

Amanda J. Morris, Alvin Li, Lindsay Jackson, Yvonne C. W. Yau², Valerie Waters³

¹Translational Medicine,Hospital for Sick Children, ²Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, ³Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Chapters

00:00Introduction
00:27Confocal Microscopy Imaging and COMSTAT Analysis
04:21Results: Quantification of Antimicrobial Effects on In Vitro Biofilm Architecture
05:49Conclusion

Summary

Automatic Translation

抗菌诱导的 铜绿假单胞 菌生物膜结构变化在从囊性纤维化和慢性肺部感染患者培养的临床分离株中有所不同。在共聚焦显微镜之后，COMSTAT软件可用于量化单个分离株的生物膜结构（例如，表面积、厚度、生物量）的变化，以评估抗感染剂的功效。

Tags

Biofilm Antimicrobial Pseudomonas Aeruginosa COMSTAT Cystic Fibrosis Tobramycin Anti-Psl Monoclonal Antibody Image Segmentation Biofilm Architecture Confocal Microscopy

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