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Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

DOI:
10.3791/61759-v

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06:18 min

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December 14, 2020

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, , , ,
1Translational Medicine,Hospital for Sick Children, 2Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, 3Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Chapters

  • 00:00Introduction
  • 00:27Confocal Microscopy Imaging and COMSTAT Analysis
  • 04:21Results: Quantification of Antimicrobial Effects on In Vitro Biofilm Architecture
  • 05:49Conclusion

Summary

Automatic Translation

Automatic Translation

Antimicrobial-induced changes to Pseudomonas aeruginosa biofilm architecture differ among clinical isolates cultured from patients with cystic fibrosis and chronic pulmonary infection. Following confocal microscopy, COMSTAT software can be utilized to quantify variations in biofilm architecture (e.g., surface area, thickness, biomass) for individual isolates to assess the efficacy of anti-infective agents.

Tags

BiofilmAntimicrobialPseudomonas AeruginosaCOMSTATCystic FibrosisTobramycinAnti-Psl Monoclonal AntibodyImage SegmentationBiofilm ArchitectureConfocal Microscopy

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