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Immunology and Infection
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マウス腹腔内脂肪貯蔵所からの脂肪形成性および線維炎症性間質細胞亜集団の単離
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
Please note that all translations are automatically generated.
Click here for the English version.
マウス腹腔内脂肪貯蔵所からの脂肪形成性および線維炎症性間質細胞亜集団の単離
DOI:
10.3791/61610-v
•
06:50 min
•
August 16, 2020
•
Julia Peics*
1
,
Lavanya Vishvanath*
2
,
Qianbin Zhang
,
Bo Shan
,
Thomas Å. Pedersen
,
Rana K. Gupta
1
Diabetes Pharmacology
,
Novo Nordisk A/S
,
2
Touchstone Diabetes Center, Department of Internal Medicine
,
University of Texas Southwestern Medical Center
Chapters
00:00
Introduction
00:35
Adipocyte Precursor Cell (APC) and Fibro‐Inflammatory Progenitor (FIP) FACS Isolation
02:22
Adipogenic and Non‐Adipogenic Cell Fraction Immunomagnetic Bead Separation
04:34
Cell Culture and Differentiation
05:07
Results: Isolation and Differentiation of Adipogenic Stromal Cell Subset
06:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、蛍光活性化細胞選別または免疫磁気ビーズ分離により、マウス腹腔内白色脂肪組織(WAT)デポから脂肪形成および線維炎症性間質細胞亜集団を単離するための技術的アプローチを説明しています。
Tags
Immunology and Infection
Stromal Cells
SVF
PDGFR
FIPs
APCs
FACS
Immunomagnetic Beads
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